人线粒体60-kd热休克蛋白单克隆抗体的研制及其在类风湿关节炎中该蛋白表达研究中的应用。
The development of monoclonal antibodies to the human mitochondrial 60-kd heat-shock protein, and their use in studying the expression of the protein in rheumatoid arthritis.
作者信息
Sharif M, Worrall J G, Singh B, Gupta R S, Lydyard P M, Lambert C, McCulloch J, Rook G A
机构信息
Department of Medical Microbiology, University College of Middlesex School of Medicine, London, United Kingdom.
出版信息
Arthritis Rheum. 1992 Dec;35(12):1427-33. doi: 10.1002/art.1780351205.
OBJECTIVE
To assess the claim that the human 60-kd heat-shock protein (HSP) is highly expressed in the joints of patients with rheumatoid arthritis (RA), but is not readily detected in normal tissues.
METHODS
Monoclonal antibodies were raised against the human 60-kd mitochondrial heat-shock protein (P1 protein; hsp60), and their specificity was established. They were then applied to synovial tissue.
RESULTS
HSP was expressed similarly in normal, osteoarthritic, and RA synovium. Low levels of hsp60 were detected in synovial fluid by immunoprecipitation.
CONCLUSION
Minor differences in the distribution of hsp60 in synovium from RA joints were attributable to increased cellularity and to the disorganization of the tissue architecture.
目的
评估关于人类60-kd热休克蛋白（HSP）在类风湿性关节炎（RA）患者关节中高表达，但在正常组织中不易检测到这一说法。
方法
制备针对人类60-kd线粒体热休克蛋白（P1蛋白；hsp60）的单克隆抗体，并确定其特异性。然后将其应用于滑膜组织。
结果
HSP在正常、骨关节炎和RA滑膜中的表达相似。通过免疫沉淀在滑液中检测到低水平的hsp60。
结论
RA关节滑膜中hsp60分布的微小差异归因于细胞增多和组织结构紊乱。
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