Sato H, Miyata M, Kasukawa R
Department of Internal Medicine II, Fukushima Medical College, Japan.
J Rheumatol. 1996 Dec;23(12):2027-32.
To determine whether heat shock proteins (HSP) are expressed on the surface of peripheral blood (PB) lymphocytes and synovial fluid (SF) lymphocytes from patients with rheumatoid arthritis (RA).
Immunoprecipitation and flow cytometric analyses using anti-Yersinia enterocolitica HSP60 monoclonal antibodies (Mab) were performed. Phenotypes of surface HSP-positive lymphocytes were analyzed further by 2 color flow cytometry.
Anti-Y. enterocolitica HSP60 Mab immunoprecipitated 120, 97, 70, 60, 55, and 50 kDa proteins in extracts of healthy donor PB lymphocytes and of Daudi cells after thermal shock. Among these proteins, the 60 kDa HSP showed the greatest degree of precipitation. Surface HSP-positive lymphocytes were detected in 2 of 16 PB and 1 of 12 SF lymphocyte samples. Longitudinal studies indicated that HSP were expressed on the surface of lymphocytes in the active stage, but not in the improvement stage of the disease. Surface HSP-positive lymphocytes were mainly either [gamma delta +] T cells or [CD19+] B cells.
HSP were expressed on the surface of PB and SF lymphocytes from patients with RA. Surface HSP-positive lymphocytes might be recognized by lymphocytes since HSP are strongly immunogenic.
确定热休克蛋白（HSP）是否在类风湿关节炎（RA）患者的外周血（PB）淋巴细胞和滑液（SF）淋巴细胞表面表达。
采用抗小肠结肠炎耶尔森菌HSP60单克隆抗体（Mab）进行免疫沉淀和流式细胞术分析。通过双色流式细胞术进一步分析表面HSP阳性淋巴细胞的表型。
抗小肠结肠炎耶尔森菌HSP60 Mab在热休克后健康供体PB淋巴细胞和Daudi细胞提取物中免疫沉淀出120、97、70、60、55和50 kDa的蛋白。在这些蛋白中，60 kDa的HSP沉淀程度最高。在16份PB淋巴细胞样本中的2份和12份SF淋巴细胞样本中的1份中检测到表面HSP阳性淋巴细胞。纵向研究表明，HSP在疾病的活动期在淋巴细胞表面表达，但在缓解期不表达。表面HSP阳性淋巴细胞主要是[γδ +] T细胞或[CD19 +] B细胞。
HSP在RA患者的PB和SF淋巴细胞表面表达。由于HSP具有强免疫原性，表面HSP阳性淋巴细胞可能被淋巴细胞识别。
