黄酮类化合物对HL-60细胞中DCF生成的抗氧化作用。
Antioxidant effect of flavonoids on DCF production in HL-60 cells.
作者信息
Takamatsu Satoshi, Galal Ahmed M, Ross Samir A, Ferreira Daneel, ElSohly Mahmoud A, Ibrahim Abdel-Rahim S, El-Feraly Farouk S
机构信息
National Center for Natural Products Research and Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA.
出版信息
Phytother Res. 2003 Sep;17(8):963-6. doi: 10.1002/ptr.1289.
A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL-60 by assessing oxidation of 2',7'-dichloro fluorescin diacetate (DCFH-DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. In the present study, the antioxidant activity of a number of structurally related flavonoids of plant origin and some of their microbial transformation products (1-18) were evaluated.
通过评估2',7'-二氯荧光素二乙酸酯（DCFH-DA）的氧化情况，采用荧光微孔板分析法检测人白血病细胞系HL-60中的呼吸爆发活性。该方法基于检测由于过氧化氢的存在而导致的DCFH氧化。在本研究中，评估了多种植物来源的结构相关黄酮类化合物及其一些微生物转化产物（1-18）的抗氧化活性。
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