Sakai A, Shimizu Y, Hishinuma F
Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Appl Microbiol Biotechnol. 1990 Jun;33(3):302-6. doi: 10.1007/BF00164526.
Distribution of a delta (delta) sequence of the Ty element on a chromosome of the yeast Saccharomyces cerevisiae was analysed by pulsed-field gel electrophoresis. More than 100 copies of the delta sequence were nonrandomly distributed on the chromosome. Using the delta sequence as a recombination site, mouse alpha-amylase and human beta-endorphin genes were introduced into the chromosomal DNA. The integration occurred on a particular chromosome in each case and the copy number was estimated as three to five. It was also found that single- or multi-copy integration occurred at a single or multiple sites on the particular chromosome. The integrants secreted alpha-amylase and beta-endorphin by three-to fivefold compared with single-copy integrants. This type of integration was mitotically stable over a period of 50 generations under non-selective conditions.
通过脉冲场凝胶电泳分析了酵母酿酒酵母染色体上Ty元件的δ（delta）序列的分布。超过100个δ序列拷贝非随机分布在染色体上。以δ序列作为重组位点，将小鼠α-淀粉酶和人β-内啡肽基因导入染色体DNA。在每种情况下，整合都发生在特定染色体上，估计拷贝数为三到五个。还发现单拷贝或多拷贝整合发生在特定染色体的单个或多个位点上。与单拷贝整合体相比，整合体分泌的α-淀粉酶和β-内啡肽增加了三到五倍。在非选择性条件下，这种类型的整合在50代的时间内有丝分裂稳定。
