Nakanishi K, Hirose S, Yoshimoto T, Ishizashi H, Hiroishi K, Tanaka T, Kono T, Miyasaka M, Taniguchi T, Higashino K
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3551-5. doi: 10.1073/pnas.89.8.3551.
Substantial proportions of resting B cells constitutively express low levels of IL-2 receptor (IL-2R) alpha and/or beta chains. The expression of these chains is differentially regulated by anti-IgM and IL-2/IL-4. The anti-IgM induces IL-2R alpha chain expression, whereas each of the two cytokines induces IL-2R beta chain expression in a dose-dependent manner. Moreover, IL-2 induces the growth of B cells, when the cells were pretreated with IL-2 or IL-4 for 24 h. The magnitude of this IL-2-driven B-cell growth depends upon the level of IL-2R beta chain expression. Costimulation of the B cells with IL-2 and anti-IgM shifts the dose-response curve, and the cells proliferate at an IL-2 concentration as low as 40 pM. These results indicate that the levels of anti-IgM-induced IL-2R alpha chain and IL-2-induced IL-2R beta chain determine the sensitivity of the cells to IL-2.
相当比例的静息B细胞组成性地表达低水平的白细胞介素2受体（IL-2R）α链和/或β链。这些链的表达受抗IgM和IL-2/IL-4的差异调节。抗IgM诱导IL-2Rα链表达，而这两种细胞因子中的每一种都以剂量依赖的方式诱导IL-2Rβ链表达。此外，当细胞用IL-2或IL-4预处理24小时后，IL-2诱导B细胞生长。这种由IL-2驱动的B细胞生长的程度取决于IL-2Rβ链的表达水平。用IL-2和抗IgM对B细胞进行共刺激会使剂量反应曲线发生偏移，并且细胞在低至40 pM的IL-2浓度下增殖。这些结果表明，抗IgM诱导的IL-2Rα链水平和IL-2诱导的IL-2Rβ链水平决定了细胞对IL-2的敏感性。
