NADPH氧化酶相关的H⁺通道可被花生四烯酸盐打开。
The NADPH-oxidase-associated H+ channel is opened by arachidonate.
作者信息
Henderson L M, Chappell J B
机构信息
Department of Biochemistry, School of Medical Sciences, University of Bristol, U.K.
出版信息
Biochem J. 1992 Apr 1;283 ( Pt 1)(Pt 1):171-5. doi: 10.1042/bj2830171.
Abstract
The H+ channel associated with the generation of O2.- by NADPH oxidase and the oxidase itself must both be activated in response to stimuli (e.g. phorbol esters, chemotactic peptides, certain fatty acids). We have investigated the effects of membrane potential, an imposed pH gradient and a combination of the two (the protonmotive force) on the H+ conductivity of the cytoplast membrane. H+ conductivity was observed only in the presence of arachidonate and not in its absence. In the presence of arachidonate, H+ movement was determined by the protonmotive force. The effect of arachidonate was probably on a channel, since this fatty acid did not significantly increase the H+ permeability of artificial phospholipid membranes. It appears, therefore, that arachidonate is required both for the activation of O2.- production and the associated H(+)-channel-mediated efflux.
摘要
与NADPH氧化酶产生超氧阴离子（O2.-）相关的H+通道以及氧化酶本身都必须响应刺激（如佛波酯、趋化肽、某些脂肪酸）而被激活。我们研究了膜电位、外加pH梯度以及两者的组合（质子动力）对细胞质膜H+电导率的影响。仅在花生四烯酸存在时观察到H+电导率，不存在时则未观察到。在花生四烯酸存在的情况下，H+的移动由质子动力决定。花生四烯酸的作用可能是作用于一个通道，因为这种脂肪酸并未显著增加人工磷脂膜的H+通透性。因此，似乎花生四烯酸对于超氧阴离子（O2.-）生成的激活以及相关的H(+)-通道介导的外流都是必需的。
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