SCHWAB J H, CROMARTIE W J
J Exp Med. 1960 Mar 1;111(3):295-307. doi: 10.1084/jem.111.3.295.
A macromolecular complex of C polysaccharide and peptide derived from Group A streptococcal cells produces a multinodular, remittent, and intermittent lesion of dermal connective tissue following a single intradermal injection. This lesion is greatly modified, both in the gross and microscopically, in rabbits hyperimmunized with a Group A streptococcal vaccine. The immune serum, absorbed to yield only antibodies against C polysaccharide as indicated by agar diffusion techniques, will precipitate and neutralize the toxic material. This neutralization and in vitro precipitation can be inhibited with formamide-isolated C polysaccharide hapten and with N-acetylglucosamine, the primary directive group of this antigen. This clearly demonstrates that antibodies against the group-specific C polysaccharide are responsible for resistance to this toxic material. The immunological and other properties of the toxic C polysaccharide complex, the fact that it is a product of Group A streptococci, the gross and microscopic features of the experimental lesion produced with this substance, and the observations on the chronic course of the experimental lesion, indicate that this toxic material should be investigated as a possible factor in the pathogenesis of the non-suppurative sequelae associated with Group A streptococci.
从A组链球菌细胞中提取的C多糖和肽的大分子复合物，在单次皮内注射后会引起真皮结缔组织的多结节性、弛张性和间歇性病变。在经A组链球菌疫苗高度免疫的兔子中，这种病变在大体和显微镜下都有很大改变。通过琼脂扩散技术表明，经吸收后仅产生针对C多糖抗体的免疫血清，会沉淀并中和有毒物质。这种中和作用和体外沉淀可被甲酰胺分离的C多糖半抗原以及该抗原的主要指示基团N-乙酰葡糖胺所抑制。这清楚地表明，针对组特异性C多糖的抗体是抵抗这种有毒物质的原因。有毒C多糖复合物的免疫学及其他特性、它是A组链球菌产物这一事实、用该物质产生的实验性病变的大体和显微镜特征，以及对实验性病变慢性病程的观察，表明这种有毒物质应作为与A组链球菌相关的非化脓性后遗症发病机制中的一个可能因素进行研究。
