Ovelgönne J H, Bol A W, Hop W C, van Wijk R
Department of Molecular Cell Biology, State University of Utrecht, The Netherlands.
Experientia. 1992 May 15;48(5):504-8. doi: 10.1007/BF01928175.
A previously reported effect of mechanically agitated dilutions of antiserum raised against IgE was investigated using the loss of metachromatic staining properties of human basophil leukocytes as a model. A series of 24 blind experiments was performed in which we determined the number of toluidine blue-stainable basophils after incubating with vortexed or non-vortexed dilutions of anti-IgE. Tenfold serial dilutions were used, in the range 10(21) to 10(30) (6.6 x 10(-26) to 6.6 x 10(-35) M anti-IgE). We found no evidence for a different effect of strongly agitated dilutions, compared to dilutions made with minimal physical agitation. In fact, in our hands no effect of extreme dilutions was shown at all. We conclude that the effect of extreme dilutions of anti-IgE, reported by Davenas et al., needs further clarification and that in this process the reproducibility of results between experimenters should be carefully determined.
以人嗜碱性白细胞异染性染色特性的丧失为模型，对先前报道的抗IgE抗血清机械搅拌稀释的效应进行了研究。进行了一系列24次盲法实验，在实验中我们测定了与经涡旋或未经涡旋的抗IgE稀释液孵育后甲苯胺蓝可染色嗜碱性粒细胞的数量。使用了10倍系列稀释，范围为10（-21）至10（-30）（6.6×10（-26）至6.6×10（-35）M抗IgE）。与极少物理搅拌制备的稀释液相比，我们没有发现强烈搅拌稀释液有不同效应的证据。事实上，在我们的实验中根本没有显示出极端稀释的效应。我们得出结论，Davenas等人报道的抗IgE极端稀释效应需要进一步阐明，并且在此过程中应仔细确定实验者之间结果的可重复性。
