Muramoto T, Kitamoto T, Tateishi J, Goto I
Department of Neuropathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Am J Pathol. 1992 Jun;140(6):1411-20.
The distribution and sequential development of prion protein (PrP) accumulation in the central nervous system (CNS) and non-neuronal organs of mice infected with Creutzfeldt-Jakob disease (CJD) were investigated immunohistochemically using a new pretreatment method that greatly enhanced the immunoreactivity of PrP. Prion protein accumulation in the CNS was first detected at 30 days after inoculation and then developed near the inoculation site or periventricular area, and later spread to the whole cerebrum and then to the pons. Its staining took some characteristic forms. Among non-neuronal organs, PrP accumulated in the follicular dendritic cells (FDCs) in spleen, lymph node, Peyer's patch, and thymus. FDCs staining appeared in spleen, lymph node, and Peyer's patch at 21 or 30 days after inoculation, and in thymus at 90 days. Germinal centers developed in the thymus of some CJD-infected mice. No PrP staining was detected in any examined organs of age-matched control mice.
采用一种能显著增强朊病毒蛋白（PrP）免疫反应性的新预处理方法，通过免疫组织化学研究了感染克雅氏病（CJD）的小鼠中枢神经系统（CNS）和非神经器官中PrP积累的分布及顺序发展情况。接种后30天首次在CNS中检测到PrP积累，随后在接种部位附近或脑室周围区域出现，之后扩散至整个大脑，再到脑桥。其染色呈现出一些特征性形态。在非神经器官中，PrP在脾脏、淋巴结、派伊尔结和胸腺的滤泡树突状细胞（FDCs）中积累。接种后21天或30天，脾脏、淋巴结和派伊尔结出现FDCs染色，90天胸腺出现染色。一些感染CJD的小鼠胸腺中出现生发中心。在年龄匹配的对照小鼠的任何检测器官中均未检测到PrP染色。
