Muramoto T, Kitamoto T, Tateishi J, Goto I
Department of Neuropathology, Faculty of Medicine, Kyushu University 60, Fukuoka, Japan.
Am J Pathol. 1993 Nov;143(5):1470-9.
We immunohistochemically studied the location of abnormal prion protein in the central nervous system and visceral organs at the clinical and preclinical stages of mice infected with Creutzfeldt-Jakob disease via intraperitoneal route. Abnormal prion protein was diffusely distributed in the central nervous system. The sequential study showed that its stainings were first detected 120 days after inoculation, were found in all mice after 180 days, and were the most intense and widespread after 270 days. There was no restricted involvement at the early stages nor rostrally dominant distribution of the stainings that had been found in mice infected via intracerebral route. Abnormal prion protein was also located in the follicular dendritic cells in the spleen, lymph nodes, intestinal Peyer's patch, and thymus. Its stainings were first detected in the spleen, lymph nodes, and Peyer's patch 14 or 30 days after inoculation. In the thymus, however, the stainings were first detected after 210 days in the germinal centers formed in the medulla.
我们通过免疫组化方法研究了经腹腔途径感染克雅氏病的小鼠在临床和临床前期阶段中枢神经系统及内脏器官中异常朊蛋白的定位。异常朊蛋白在中枢神经系统中呈弥漫性分布。序贯研究表明，接种后120天首次检测到其染色，180天后在所有小鼠中均能发现，270天后染色最为强烈且分布最为广泛。早期没有局限性受累，也没有像经脑内途径感染的小鼠那样出现染色的向头端优势分布。异常朊蛋白也位于脾脏、淋巴结、肠道派尔集合淋巴结和胸腺的滤泡树突状细胞中。接种后14天或30天在脾脏、淋巴结和派尔集合淋巴结中首次检测到其染色。然而，在胸腺中，染色在髓质形成的生发中心中于210天后首次检测到。
