Kronborg G, Fomsgaard A, Galanos C, Freudenberg M A, Høiby N
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
J Clin Microbiol. 1992 Jul;30(7):1848-55. doi: 10.1128/jcm.30.7.1848-1855.1992.
Enzyme-linked immunosorbent assays were developed separately for the three main parts of the Pseudomonas aeruginosa lipopolysaccharide (LPS) molecule, namely, lipid A, core, and O polysaccharide. Anti-lipid A, anticore, and anti-O polysaccharide antibodies were measured in serum samples from 12 patients with cystic fibrosis (CF) in a longitudinal study covering the period before P. aeruginosa infection was established through at least 5 years of chronic infection. The serum antibody response to all parts of the P. aeruginosa LPS molecule increased during the course of chronic infection. The increase in anti-lipid A antibodies was specific for P. aeruginosa lipid A, since no increase in anti-Escherichia coli lipid A antibodies was seen. Immunoglobulin G, A, and M (IgG, IgA and IgM) antibodies were all involved in the specific systemic response to P. aeruginosa lipid A, core, and the O polysaccharides. IgG and IgA levels in particular increased during the course of infection and were significantly higher than the antibody increase seen with age in a healthy control group. The local immune response in the lungs was investigated by measuring IgG, IgA, and IgM antibodies to the separate parts of the P. aeruginosa LPS molecule in sputum samples from 18 CF patients with at least a 5-year history of chronic P. aeruginosa infection. Antibodies detected in sputum were mainly anti-lipid A and anti-O polysaccharide antibodies of the IgG and IgA isotypes. Very high IgA anti-lipid A titers were detected in sputum samples from some CF patients.
针对铜绿假单胞菌脂多糖（LPS）分子的三个主要部分，即脂质A、核心多糖和O多糖，分别开发了酶联免疫吸附测定法。在一项纵向研究中，对12名囊性纤维化（CF）患者的血清样本中的抗脂质A、抗核心多糖和抗O多糖抗体进行了检测，该研究涵盖了从铜绿假单胞菌感染确立前到至少5年慢性感染的时间段。在慢性感染过程中，血清对铜绿假单胞菌LPS分子所有部分的抗体反应均增强。抗脂质A抗体的增加对铜绿假单胞菌脂质A具有特异性，因为未观察到抗大肠杆菌脂质A抗体的增加。免疫球蛋白G、A和M（IgG、IgA和IgM）抗体均参与了对铜绿假单胞菌脂质A、核心多糖和O多糖的特异性全身反应。尤其是IgG和IgA水平在感染过程中升高，且显著高于健康对照组中随年龄增长而出现的抗体增加。通过检测18名有至少5年铜绿假单胞菌慢性感染病史的CF患者痰液样本中针对铜绿假单胞菌LPS分子各部分的IgG、IgA和IgM抗体，研究了肺部的局部免疫反应。痰液中检测到的抗体主要是IgG和IgA亚型的抗脂质A和抗O多糖抗体。在一些CF患者的痰液样本中检测到非常高的IgA抗脂质A滴度。
