血清阳性血清对1型人类免疫缺陷病毒逆转录酶热失活的抑制作用。
Inhibition of heat inactivation of reverse transcriptase of human immunodeficiency virus type 1 by seropositive sera.
作者信息
Nagumo T, Takeuchi Y, Hoshino H
机构信息
Department of Hygiene and Virology, Gunma University School of Medicine.
出版信息
Jpn J Cancer Res. 1992 Jun;83(6):594-600. doi: 10.1111/j.1349-7006.1992.tb00131.x.
Abstract
The reverse transcriptase (RT) activity of a lysate of human immunodeficiency virus type 1 (HIV) was almost completely inactivated by incubation at 56 degrees C for 20-30 min. The heat-inactivation of RT in the virus lysate or purified RT was partially inhibited in the presence of some human sera or plasma containing antibodies against HIV. The IgG fraction purified from the seropositive sera was responsible for stabilization of RT upon heat inactivation. This is a new assay system for detection of antibodies against RT.
摘要
1型人类免疫缺陷病毒（HIV）裂解物的逆转录酶（RT）活性在56℃孵育20 - 30分钟后几乎完全失活。在含有抗HIV抗体的一些人血清或血浆存在的情况下，病毒裂解物或纯化的RT中RT的热失活受到部分抑制。从血清阳性血清中纯化的IgG组分在热失活时负责RT的稳定。这是一种检测抗RT抗体的新检测系统。
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