Wilkinson J M, Galea-Lauri J, Sellars R A, Boniface C
Department of Biochemistry and Cell Biology, Hunterian Institute, Royal College of Surgeons, London, U.K.
Immunology. 1992 Aug;76(4):625-30.
Three monoclonal antibodies which recognize rabbit leucocytes have been characterized by immunofluorescence staining of a variety of cell populations and also by immunochemical techniques. The evidence obtained suggests that these antibodies recognize the rabbit equivalents of the CD58/LFA-3 (VC21), CD43/leukosialin (L11/135) and CD9 (MM2/57) antigens. A fourth antibody, RPN3/57, recognizes an antigen expressed strongly on T cells, thymocytes and neutrophils and at lower levels on platelets. It has not, however been possible to characterize the antigen recognized by RPN3/57 in molecular terms. Both L11/135 and RPN3/57 are useful reagents for the detection of T cells both by flow cytometry and by immunohistochemistry.
三种识别兔白细胞的单克隆抗体已通过对多种细胞群体的免疫荧光染色以及免疫化学技术进行了表征。获得的证据表明，这些抗体识别兔体内与CD58/LFA-3（VC21）、CD43/白细胞涎酸蛋白（L11/135）和CD9（MM2/57）抗原相对应的物质。第四种抗体RPN3/57识别一种在T细胞、胸腺细胞和中性粒细胞上强烈表达且在血小板上表达水平较低的抗原。然而，尚未能够从分子层面表征RPN3/57所识别的抗原。L11/135和RPN3/57都是通过流式细胞术和免疫组织化学检测T细胞的有用试剂。
