检测到阳性BacT/Alert血培养的时间以及无需对5至7天的阴性培养物进行常规传代培养。
Time to detection of positive BacT/Alert blood cultures and lack of need for routine subculture of 5- to 7-day negative cultures.
作者信息
Hardy D J, Hulbert B B, Migneault P C
机构信息
Department of Microbiology and Immunology, University of Rochester, Medical Center, New York 14642-8710.
出版信息
J Clin Microbiol. 1992 Oct;30(10):2743-5. doi: 10.1128/jcm.30.10.2743-2745.1992.
Abstract
Consecutive BacT/Alert blood cultures which were instrument negative following a 7-day incubation were subcultured. Eighteen (0.2%) of 11,476 bottles had growth on subculture. Eleven of these eighteen isolates were considered contaminants on the basis of the identity of the organism and lack of other positive blood cultures from the same patient. In addition, analysis of time to instrument detection for approximately 2,900 positive blood cultures indicates that 5 or 6 days of incubation is sufficient for the routine detection of clinically significant organisms from BacT/Alert blood cultures. These data indicate that subculture of 5- to 7-day instrument-negative BacT/Alert blood culture bottles is not necessary.
摘要
对连续培养7天后仪器检测呈阴性的BacT/Alert血培养物进行传代培养。11476瓶中有18瓶（0.2%）传代培养时有生长。根据分离出的微生物种类以及同一患者其他血培养结果均为阴性，这18株分离菌中有11株被认为是污染物。此外，对约2900份阳性血培养物的仪器检测时间分析表明，培养5或6天足以从BacT/Alert血培养物中常规检测出具有临床意义的微生物。这些数据表明，对培养5至7天仪器检测呈阴性的BacT/Alert血培养瓶进行传代培养没有必要。
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