Wright R J, Carne A, Hieber A D, Lamont I L, Emerson G W, Sullivan P A
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
J Bacteriol. 1992 Dec;174(23):7848-53. doi: 10.1128/jb.174.23.7848-7853.1992.
The gene (PRA11) encoding a secreted aspartate proteinase of Candida albicans has been cloned and sequenced. The nucleotide and deduced amino acid sequences of PRA11 are 77 and 73% identical, respectively, with the reported sequences of PRA10 also cloned from C. albicans. Southern analyses indicated that the genome of each strain examined (ATCC 10231 and ATCC 10261) contains PRA10 and PRA11. Northern (RNA) analyses showed that PRA11 was expressed at a much higher level than was PRA10 when secretion of the proteinase by strain ATCC 10261 was induced with albumin.
编码白色念珠菌分泌型天冬氨酸蛋白酶的基因（PRA11）已被克隆和测序。PRA11的核苷酸序列和推导的氨基酸序列分别与同样从白色念珠菌中克隆得到的PRA10的报道序列有77%和73%的同源性。Southern分析表明，所检测的每个菌株（ATCC 10231和ATCC 10261）的基因组都含有PRA10和PRA11。Northern（RNA）分析显示，当用白蛋白诱导ATCC 10261菌株分泌蛋白酶时，PRA11的表达水平比PRA10高得多。
