Magee B B, Hube B, Wright R J, Sullivan P J, Magee P T
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.
Infect Immun. 1993 Aug;61(8):3240-3. doi: 10.1128/iai.61.8.3240-3243.1993.
The secreted aspartyl proteinase activity from Candida albicans is thought to be a potential virulence factor. Four laboratories have cloned a gene from C. albicans encoding this enzyme. When two of these genes sharing 77% homology at the DNA level are hybridized under conditions of high stringency to contour-clamped homogeneous electric field chromosome separations of four different strains, they label different chromosomes: chromosome 6 for SAP1 and chromosome R for SAP2. The existence of different genes for the two sequences was confirmed by polymerase chain reaction. Genomic Southern blots probed with the genes and washed at low stringency revealed several cross-hybridizing bands. Contour-clamped homogeneous electric field chromosome separations probed at low stringency indicated that there was a cross-hybridizing sequence on chromosome 3 in addition to those on chromosomes R and 6. The genes for the secreted aspartyl proteinase activity in C. albicans thus constitute a gene family which we have called the SAP family.
白色念珠菌分泌的天冬氨酸蛋白酶活性被认为是一种潜在的致病因素。四个实验室已从白色念珠菌中克隆出编码该酶的基因。当其中两个在DNA水平上具有77%同源性的基因在高严格条件下与四种不同菌株的轮廓夹闭均匀电场染色体分离物杂交时，它们标记不同的染色体：SAP1标记6号染色体，SAP2标记R号染色体。通过聚合酶链反应证实了这两个序列存在不同的基因。用这些基因进行探测并在低严格条件下洗涤的基因组Southern印迹显示出几条交叉杂交带。在低严格条件下探测的轮廓夹闭均匀电场染色体分离表明，除了R号和6号染色体上的序列外，3号染色体上还有一个交叉杂交序列。因此，白色念珠菌中分泌天冬氨酸蛋白酶活性的基因构成了一个我们称为SAP家族的基因家族。
