Terpstra A H M, Javadi M, Beynen A C, Kocsis S, Lankhorst A E, Lemmens A G, Mohede I C M
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584 CM Utrecht, The Netherlands.
J Nutr. 2003 Oct;133(10):3181-6. doi: 10.1093/jn/133.10.3181.
The objective of this study was to compare the effects of conjugated linoleic acid (CLA) as triacylglycerols (TAG) or free fatty acids (FFA) on body composition and energy balance in mice. We fed four groups of 5-wk-old Balb-C mice (n = 9) semipurified diets containing either CLA (0.5 g CLA/100 g of diet) or high oleic sunflower oil (HOSF) in the form of FFA or TAG for 42 d. Body composition was determined and the energy in the carcasses, excreta and food was measured in a bomb calorimeter. The amount of body fat was 4.72 +/- 0.95 g (17.9 +/- 2.8%) in the HOSF-FFA group, 2.36 +/- 0.29 g (9.4 +/- 1.0%) in the CLA-FFA mice (mean +/- SD, P < 0.05), 4.76 +/- 0.74 g (18.2 +/- 2.2%) in the HOSF-TAG group and 2.32 +/- 0.38 g (9.3 +/- 1.1%) in the CLA-TAG mice (P < 0.05). The percentage of energy intake that was stored in the body was 3.5 +/- 1.2% in the HOSF-FFA group, 0.6 +/- 0.3% in the CLA-FFA group (P < 0.05), 3.5 +/- 1.1% in the HOSF-TAG group and 0.5 +/- 0.4 in the CLA-TAG mice (P < 0.05). Conversely, the percentage of energy intake that was expended as heat was 89.4 +/- 1.2% in the HOSF-FFA group, 92.4 +/- 0.8% in the CLA-FFA mice (P < 0.05), 89.47 +/- 1.23% in the HOSF-TAG group and 92.2 +/- 0.4% in the CLA-TAG group (P < 0.05). Thus, CLA in the form of FFA or TAG had similar effects on body composition and energy balance.
本研究的目的是比较共轭亚油酸（CLA）以三酰甘油（TAG）或游离脂肪酸（FFA）形式对小鼠身体组成和能量平衡的影响。我们给四组5周龄的Balb-C小鼠（n = 9）喂食半纯化日粮，日粮中分别含有CLA（0.5 g CLA/100 g日粮）或高油酸葵花籽油（HOSF），其形式为FFA或TAG，持续42天。测定身体组成，并在弹式量热计中测量 carcasses、排泄物和食物中的能量。HOSF - FFA组的体脂量为4.72±0.95 g（17.9±2.8%），CLA - FFA小鼠组为2.36±0.29 g（9.4±1.0%）（平均值±标准差，P < 0.05），HOSF - TAG组为4.76±0.74 g（18.2±2.2%），CLA - TAG小鼠组为2.32±0.38 g（9.3±1.1%）（P < 0.05）。HOSF - FFA组中储存于体内的能量摄入百分比为3.5±1.2%，CLA - FFA组为0.6±0.3%（P < 0.05），HOSF - TAG组为3.5±1.1%，CLA - TAG小鼠组为0.5±0.4（P < 0.05）。相反，以热量形式消耗的能量摄入百分比在HOSF - FFA组为89.4±1.2%，CLA - FFA小鼠组为92.4±0.8%（P < 0.05），HOSF - TAG组为89.47±1.23%，CLA - TAG组为92.2±0.4%（P < 0.05）。因此，FFA或TAG形式的CLA对身体组成和能量平衡具有相似的影响。
