Felix R, Fleisch H, Frandsen P L
Department of Pathophysiology, University of Berne, Switzerland.
Infect Immun. 1992 Dec;60(12):4984-8. doi: 10.1128/iai.60.12.4984-4988.1992.
Pasteurella multocida toxin (PMT), which is the primary etiologic factor in the pathogenesis of progressive atrophic rhinitis in pigs, was found to stimulate bone resorption in vitro. This stimulation was observed both in cultures of murine calvaria by measuring the release of calcium and of the lysosomal enzyme beta-glucuronidase and in murine long bone cultures by measuring the release of calcium. Both systems showed the same dose response curve, with the maximal effect at a concentration of 5 ng/ml. The effect on calvaria was studied in more detail. PMT increased bone resorption 24 h after its addition and always had to be present to express an effect. Calcitonin was able to inhibit this increase of resorption completely, and inhibitors of prostaglandin synthesis suppressed it partially. Although the data show an effect of PMT on bone tissue, the results do not exclude an action on cells in the nasal cavity, which could indirectly stimulate bone resorption.
多杀巴斯德菌毒素（PMT）是猪进行性萎缩性鼻炎发病机制中的主要病因，已发现其在体外可刺激骨吸收。通过测量钙和溶酶体酶β-葡萄糖醛酸酶的释放，在小鼠颅骨培养物中观察到了这种刺激作用；通过测量钙的释放，在小鼠长骨培养物中也观察到了这种刺激作用。两个系统都显示出相同的剂量反应曲线，在浓度为5 ng/ml时达到最大效应。对颅骨的作用进行了更详细的研究。PMT在添加后24小时增加骨吸收，并且始终必须存在才能发挥作用。降钙素能够完全抑制这种吸收增加，前列腺素合成抑制剂则部分抑制它。尽管数据显示PMT对骨组织有作用，但结果并不排除其对鼻腔细胞有作用，而鼻腔细胞可能间接刺激骨吸收。
