Masuzawa T, Beppu Y, Kawabata H, Yanagihara Y, Iwamoto Y, Shimizu T, Johnson R C
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
J Clin Microbiol. 1992 Nov;30(11):3016-8. doi: 10.1128/jcm.30.11.3016-3018.1992.
Infectivity of Borrelia burgdorferi strain 297 in normal outbred ddY mice was examined. Strain 297 was inoculated intraperitoneally in 3-week-old outbred ddY mice. B. burgdorferi was routinely cultured from the heart and urinary bladder 5 to 84 days postinoculation. The combined isolation rate from both heart and urinary bladder was significantly higher than the rate from spleen, kidney, liver, urine, and blood samples. Three- and 10-week-old mice were infected with inocula of 10(4) and 10(5) or more, respectively. Passive transfer of undiluted and 10-fold-diluted anti-297 rabbit serum and active immunization of 50 to 100 micrograms of lyophilized whole cells completely protected mice from infection with B. burgdorferi. These results suggest that the outbred mouse is a convenient model for experimental infection with B. burgdorferi.
检测了伯氏疏螺旋体297株在正常远交ddY小鼠中的感染性。将297株接种于3周龄的远交ddY小鼠腹腔内。接种后5至84天，常规从心脏和膀胱培养出伯氏疏螺旋体。心脏和膀胱的联合分离率显著高于脾脏、肾脏、肝脏、尿液和血液样本的分离率。分别用10(4)和10(5)或更多的接种物感染3周龄和10周龄的小鼠。未稀释和10倍稀释的抗297兔血清的被动转移以及50至100微克冻干全细胞的主动免疫可完全保护小鼠免受伯氏疏螺旋体感染。这些结果表明，远交小鼠是伯氏疏螺旋体实验感染的便捷模型。
