Attin T, Meyer K, Hellwig E, Buchalla W, Lennon A M
Department of Operative and Preventive Dentistry and Periodontology, Georg-August-University Göttingen, Robert-Koch-Str. 40, D-37075, Göttingen, Germany.
Arch Oral Biol. 2003 Nov;48(11):753-9. doi: 10.1016/s0003-9969(03)00156-0.
The aim of this study was to evaluate the effect of mineral supplements to citric acid (1%; pH 2.21) on enamel erosion under controlled conditions in an artificial mouth. From each of 156 bovine incisors one polished enamel sample was prepared. The samples were divided among 13 experimental groups (n=12). In group 1 citric acid only was used (control). In groups 2-10 either calcium, phosphate or fluoride in various low concentrations was admixed to the citric acid. In groups 11-13 the citric acid was supplemented with a mixture of calcium, phosphate and fluoride. For demineralisation the specimens were rinsed with the respective solution for 1 min, immediately followed by a remineralisation period with artificial saliva (1 min). The specimens were cycled through this alternating procedure five times followed by rinsing for 8 h with artificial saliva. The de- and remineralisation cycle was repeated three times for each specimen interrupted by the 8 h-remineralisation periods. Before and after the experiments, the specimens were examined using microhardness testing (Knoop hardness) and laser profilometry. Hardness loss and enamel dissolution was significantly higher for the controls as compared to the remaining groups. Significantly lowest hardness loss for all groups was recorded for group 12 with admixture of calcium, phosphate and fluoride to citric acid. The significantly highest enamel loss was recorded for the controls compared to all other samples. Groups 3 and 4 revealed significantly lower and higher tissue loss compared to the remaining groups (2-13), respectively. The other groups did not differ significantly from each other. Modification of citric acid with calcium, phosphate and fluoride exerts a significant protective potential with respect to dental erosion. However, with the low concentrations applied enamel dissolution could not be completely prevented.
本研究的目的是在人工口腔的可控条件下，评估矿物质补充剂对柠檬酸（1%；pH 2.21）所致牙釉质侵蚀的影响。从156颗牛切牙中各制备一个经抛光的牙釉质样本。将样本分为13个实验组（n = 12）。第1组仅使用柠檬酸（对照组）。第2 - 10组在柠檬酸中混入各种低浓度的钙、磷或氟。第11 - 13组的柠檬酸中添加了钙、磷和氟的混合物。为使样本脱矿，将其在相应溶液中冲洗1分钟，随后立即进行人工唾液再矿化1分钟。样本循环进行此交替过程5次，然后用人工唾液冲洗8小时。每个样本重复脱矿和再矿化循环3次，期间穿插8小时的再矿化期。实验前后，使用显微硬度测试（努氏硬度）和激光轮廓仪对样本进行检查。与其余组相比，对照组的硬度损失和牙釉质溶解明显更高。第12组在柠檬酸中添加钙、磷和氟，记录到所有组中硬度损失显著最低。与所有其他样本相比，对照组记录到牙釉质损失显著最高。与其余组（2 - 13）相比，第3组和第4组分别显示出明显更低和更高的组织损失。其他组之间无显著差异。用钙、磷和氟对柠檬酸进行改性，对牙齿侵蚀具有显著的保护潜力。然而，由于所使用的浓度较低，无法完全防止牙釉质溶解。
