Badiou S, Dupuy A M, Descomps B, Cristolead J P
Laboratoire de Biochimie, Hopital Lapeyronie, Montpellier, France.
J Clin Lab Anal. 2003;17(6):235-40. doi: 10.1002/jcla.10103.
To evaluate the relationship between the enzymatic Vitros assay for creatinine determination and other methods, we determined creatinine concentration in 400 heparin samples. Plasma creatinine level was successively determined on the Vitros 750 analyzer (Johnson & Johnson Co., Rochester, NY) and on the Olympus AU2700 analyzer (Olympus France, Rungis, France), using three reagents in the same assay: Olympus-Jaffé and two enzymatic commercial kits-Crea Plus (Roche Diagnostics, Meylan, France) and Enzymatic Creatinine (Randox, Mauguio, France). Comparison of Jaffé and enzymatic measurements of plasma creatinine levels revealed a high correlation when considering all values ranged from 20-1000 micromol/l (r > 0.99). However, for values <60 micromol/l, enzymatic reagents provided best results. Enzymatic methodology is a better clinical choice for the accurate measurement of creatinine, especially for neonates, pediatrics, and hematology units. Because analytical performance and the costs of Randox creatinine were satisfactory for our laboratory, this method, adapted on the Olympus analyzer, was chosen for routine determination of creatinine levels. According to the Valtec protocol (8), no interferences such as hemolysis, lipemia, or bilirubin were detected for Enzymatic Creatinine Randox.
为评估用于肌酐测定的酶促Vitros检测法与其他方法之间的关系，我们测定了400份肝素样本中的肌酐浓度。在Vitros 750分析仪（强生公司，纽约州罗切斯特）和奥林巴斯AU2700分析仪（奥林巴斯法国公司，法国伦吉斯）上，使用同一项检测中的三种试剂，依次测定血浆肌酐水平：奥林巴斯-贾菲试剂以及两种酶促商业试剂盒——Crea Plus（罗氏诊断公司，法国梅兰）和酶促肌酐试剂（朗道克斯公司，法国莫吉隆）。比较血浆肌酐水平的贾菲检测法和酶促检测法发现，当考虑所有20 - 1000微摩尔/升范围内的值时，两者具有高度相关性（r > 0.99）。然而，对于<60微摩尔/升的值，酶促试剂提供了最佳结果。酶促方法对于准确测量肌酐是更好的临床选择，尤其是对于新生儿、儿科和血液科。由于朗道克斯肌酐试剂的分析性能和成本对我们实验室而言令人满意，因此选择在奥林巴斯分析仪上采用该方法来常规测定肌酐水平。根据瓦尔泰克方案（8），未检测到酶促肌酐朗道克斯试剂存在溶血、脂血或胆红素等干扰情况。
