需氧大肠杆菌细胞中ATP酶动力学的31P核磁共振测量
31P nuclear magnetic resonance measurements of ATPase kinetics in aerobic Escherichia coli cells.
作者信息
Brown T R, Ugurbil K, Shulman R G
出版信息
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5551-3. doi: 10.1073/pnas.74.12.5551.
Abstract
We have measured the in vivo unidirectional rates between the terminal phosphate of ATP and intracellular inorganic phosphate (PiIN) in aerobic suspensions of Escherichia coli cells using 31P nuclear magnetic resonance saturation transfer techniques. Typically, the measurements consisted of saturating the ATPgamma resonance and observing a 20 +/- 5% reduction in the intensity of the PiIN resonance. No saturation transfer was observed after incubation with 1 mM dicyclohexylcarbodiimide, an ATPase inhibitor. From the measured decrease in intensity of PiIN coming from saturation transfer, the apparent unimolecular rate constant of PiIN to ATP was calculated to be 0.6 +/- 0.15 sec-1.
摘要
我们使用³¹P核磁共振饱和转移技术，测量了大肠杆菌细胞需氧悬浮液中ATP末端磷酸基团与细胞内无机磷酸（PiIN）之间的体内单向速率。通常，测量包括使ATPγ共振饱和，并观察到PiIN共振强度降低20±5%。用1 mM二环己基碳二亚胺（一种ATP酶抑制剂）孵育后未观察到饱和转移。根据饱和转移导致的PiIN强度测量下降，计算出PiIN与ATP的表观单分子速率常数为0.6±0.15秒⁻¹。
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