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波兰西北部蓖麻硬蜱中伯氏疏螺旋体狭义种DNA的检测

Detection of Borrelia burgdorferi sensu lato DNA in Ixodes ricinus ticks in North-western Poland.

作者信息

Wodecka Beata

机构信息

Department of Genetics, University of Szczecin, Piastow 40B, 71-065 Szczecin, Poland.

出版信息

Ann Agric Environ Med. 2003;10(2):171-8.

PMID:14677908
Abstract

In order to estimate the risk of contracting Lyme disease in the forest areas of north-western Poland, PCR-based studies were carried out on 6,817 Ixodes ricinus ticks for infection by the spirochaete B. burgdorferi sensu lato (s.l.). The studies were performed using the primers for the fla gene, conserved for all European genospecies of B. burgdorferi s.l. Based on the incidence of B. burgdorferi s.l. DNA in I. ricinus ticks at eight sampling sites during 1998-2001, it may be concluded that a risk of contracting Lyme disease is present in the forest areas of north-western Poland. The highest risk of infection (9.4 % of infected ticks) is posed by human contact with female I. ricinus, and the risk is higher in late spring and early summer than in late summer and early autumn. The north-western part of Poland is an endemic region for B. burgdorferi s.l.

摘要

为了评估波兰西北部森林地区感染莱姆病的风险,对6817只蓖麻硬蜱进行了基于聚合酶链反应(PCR)的研究,以检测其是否感染伯氏疏螺旋体狭义种(Borrelia burgdorferi sensu lato,s.l.)。研究使用了针对fla基因的引物,该基因在所有欧洲伯氏疏螺旋体狭义种的基因种中都是保守的。根据1998 - 2001年期间八个采样点的蓖麻硬蜱中伯氏疏螺旋体狭义种DNA的发生率,可以得出结论,波兰西北部的森林地区存在感染莱姆病的风险。人类与雌性蓖麻硬蜱接触带来的感染风险最高(感染蜱的9.4%),且晚春和初夏的风险高于夏末和初秋。波兰西北部是伯氏疏螺旋体狭义种的流行地区。

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