利用 PCR-限制性片段长度多态性方法，flaB 基因作为一种分子标记，可对环境样本中的伯氏疏螺旋体进行明确鉴定。
flaB gene as a molecular marker for distinct identification of Borrelia species in environmental samples by the PCR-restriction fragment length polymorphism method.
机构信息
Department of Genetics, Szczecin University, Felczaka 3c, 71-412 Szczecin, Poland.
出版信息
Appl Environ Microbiol. 2011 Oct;77(19):7088-92. doi: 10.1128/AEM.05437-11. Epub 2011 Aug 12.
Abstract
A new protocol employing nested PCR-restriction fragment length polymorphism (RFLP) based on the flaB gene and two restriction enzymes was worked out. This protocol allows the identification of all Borrelia species transmitted by Ixodes ricinus in Europe, including Borrelia miyamotoi and 3 genetic variants of B. garinii. A dendrogram of flaB sequence similarity was in accordance with RFLP variants.
摘要
制定了一种新的方案，该方案采用基于 flaB 基因和两种限制酶的嵌套 PCR-限制性片段长度多态性（RFLP）。该方案可鉴定欧洲传播的所有莱姆病螺旋体，包括 Miyamotoi 螺旋体和伯氏疏螺旋体的 3 种遗传变异体。flaB 序列相似性的系统发育树与 RFLP 变异体一致。
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