Eur J Clin Microbiol Infect Dis. 1992 Sep;11(9):777-81.
In 1991 a multicentre survey on the prevalence of metronidazole resistance in Helicobacter pylori in vitro was carried out in 12 hospitals in 11 different European countries. The susceptibility of Helicobacter pylori to metronidazole was determined in each centre by measuring the MIC on agar with the PDM E-test (AB Biodisk) according to a standard procedure. Overall, 122 of 443 (27.5%) strains tested were resistant to metronidazole (MIC > 8 micrograms/ml). The level of resistance to metronidazole varied markedly between centres (from 7% to 49%) and was found to be substantially higher in Africans and other non-Caucasian subjects than in natives from European countries. The overall rate of resistance to metronidazole was higher in females (34.7%) than in males (23.9%), and varied according to the age group, being highest among women aged 20 to 39 (50%). Previous use of metronidazole was reported in only 16 patients, 11 of whom (68.8%) harboured resistant Helicobacter pylori strains. Although differences in the rate of metronidazole resistance in Helicobacter pylori most probably relate to variations in use of this drug in different populations, such use may frequently go unrecognized. This study emphasises the importance of monitoring the drug resistance of Helicobacter pylori on a local basis. Standardisation of the methods for testing the susceptibility of Helicobacter pylori in vitro is clearly needed for this purpose.
1991年，在11个不同欧洲国家的12家医院开展了一项关于幽门螺杆菌对甲硝唑体外耐药性患病率的多中心调查。每个中心都按照标准程序，使用PDM E-test（AB Biodisk）在琼脂上测量最低抑菌浓度（MIC），以确定幽门螺杆菌对甲硝唑的敏感性。总体而言，在检测的443株菌株中，有122株（27.5%）对甲硝唑耐药（MIC>8微克/毫升）。各中心对甲硝唑的耐药水平差异显著（从7%到49%），并且发现非洲人和其他非白种人受试者的耐药水平明显高于欧洲国家的本地人。女性对甲硝唑的总体耐药率（34.7%）高于男性（23.9%），并且根据年龄组有所不同，在20至39岁的女性中最高（50%）。仅16例患者报告曾使用过甲硝唑，其中11例（68.8%）携带耐药幽门螺杆菌菌株。尽管幽门螺杆菌对甲硝唑的耐药率差异很可能与不同人群中该药物的使用差异有关，但这种使用情况可能常常未被认识到。本研究强调了在当地监测幽门螺杆菌耐药性的重要性。为此，显然需要对体外检测幽门螺杆菌敏感性的方法进行标准化。
