Block S M
Dept of Molecular Biology and the Princeton Materials Institute at Princeton University, Princeton, NJ 08544, USA.
Trends Cell Biol. 1995 Apr;5(4):169-75. doi: 10.1016/s0962-8924(00)88982-5.
Much of our current understanding of the molecular physiology of kinesin has come from in vitro motility assays: indeed, the discovery of kinesin relied upon such assays. By marrying in vitro assays with novel instruments capable of resolving movements on the molecular scale, it has proved possible to make measurements on single motors. Such key parameters as the step size, stepping force, and force-velocity relationship for kinesin have been determined in this fashion, and should soon contribute to a molecular model for the movement of kinesin.
实际上，驱动蛋白的发现就依赖于此类分析。通过将体外分析与能够解析分子尺度运动的新型仪器相结合，已证明有可能对单个马达进行测量。驱动蛋白的步长、步进力和力-速度关系等关键参数就是通过这种方式确定的，并且很快将有助于构建驱动蛋白运动的分子模型。
