Schettgen T, Kütting B, Hornig M, Beckmann M W, Weiss T, Drexler H, Angerer J
Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen-Nuremberg, Schillerstrasse 25/29, 91054 Erlangen, Germany.
Int Arch Occup Environ Health. 2004 Apr;77(3):213-6. doi: 10.1007/s00420-003-0496-8. Epub 2004 Jan 22.
Acrylamide (AA) (CAS No 79-06-1) has most recently been identified as a food-borne toxicant generated during the heating process of starch-containing foods. It was the aim of the present study to investigate the trans-placental exposure of newborn infants to this possible human carcinogen by analysis of the specific haemoglobin adduct of AA ( N-2-carbamoylethylvaline, AAV) in the blood of mothers and the corresponding umbilical cord blood of neonates as a parameter of biochemical effects.
We investigated the blood of 11 women advanced in pregnancy (one smoker, ten non-smokers) and the corresponding umbilical cord blood of neonates for the N-terminal haemoglobin adducts of AA (AAV) and the smoking-specific adduct of acrylonitrile (CAS No 107-13-1) ( N-cyanoethylvaline, CEV). The limit of detection (LOD) was 5 pmol/g globin for AAV and 4 pmol/g globin for CEV.
AAV could be determined in all blood samples of the mothers (median 21 pmol/g globin, range 18-104 pmol/g globin) as well as in the umbilical cord blood of neonates (median 10 pmol/g globin, range 6-43 pmol/g globin). The highest values were detected in the blood of the smoking mother and her child. CEV was detected only in the blood of the smoking mother (185 pmol/g globin) and the corresponding umbilical cord blood (69 pmol/g globin).
AAV adduct levels in non-smoking mothers and neonates showed a good correlation (r=0.859). The concentration of AA adducts in the blood of neonates is approximately 50% of the adduct level found in the blood of the mother. In view of the shorter life span of neonatal erythrocytes and the lower body weight of newborn infants, the relative internal dose of AA in neonates (in microgrammes per kilogramme body weight) must be assumed to be at least equal to that of the mother. Because of the high cell-replication rates during foetal development, trans-placental exposure of neonates to AA might raise concerns. Neonates of smoking mothers take up much higher doses of AA than those of non-smoking mothers.
丙烯酰胺（AA）（化学物质登记号79 - 06 - 1）最近被确认为在含淀粉食物加热过程中产生的一种食源性毒物。本研究旨在通过分析母亲血液及新生儿相应脐带血中AA的特异性血红蛋白加合物（N - 2 - 氨甲酰基乙基缬氨酸，AAV），作为生化效应参数，来研究新生儿经胎盘暴露于这种可能的人类致癌物的情况。
我们检测了11名孕晚期女性（1名吸烟者，10名非吸烟者）的血液以及新生儿相应的脐带血，以检测AA的N端血红蛋白加合物（AAV）和丙烯腈（化学物质登记号107 - 13 - 1）的吸烟特异性加合物（N - 氰基乙基缬氨酸，CEV）。AAV的检测限为5皮摩尔/克珠蛋白，CEV的检测限为4皮摩尔/克珠蛋白。
在母亲的所有血液样本中（中位数为21皮摩尔/克珠蛋白，范围为18 - 104皮摩尔/克珠蛋白）以及新生儿的脐带血中（中位数为10皮摩尔/克珠蛋白，范围为6 - 43皮摩尔/克珠蛋白）均能检测到AAV。在吸烟母亲及其孩子的血液中检测到的数值最高。仅在吸烟母亲的血液（185皮摩尔/克珠蛋白）及其相应的脐带血（69皮摩尔/克珠蛋白）中检测到了CEV。
非吸烟母亲和新生儿的AAV加合物水平显示出良好的相关性（r = 0.859）。新生儿血液中AA加合物的浓度约为母亲血液中加合物水平的50%。鉴于新生儿红细胞寿命较短且体重较低，必须假定新生儿体内AA的相对内剂量（以每千克体重微克数计）至少与母亲相等。由于胎儿发育期间细胞复制率较高，新生儿经胎盘暴露于AA可能令人担忧。吸烟母亲的新生儿摄入的AA剂量比非吸烟母亲的新生儿高得多。
