Samoilova Kira A, Bogacheva Olga N, Obolenskaya Ksenya D, Blinova Miralda I, Kalmykova Natalya V, Kuzminikh Elena V
Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave, Saint Petersburg, 194064, Russia.
Photochem Photobiol Sci. 2004 Jan;3(1):96-101. doi: 10.1039/b305738k. Epub 2003 Sep 1.
The systemic mechanisms of the wound healing effect of low intensity lasers remain largely uninvestigated. The goal of this randomized, placebo controlled, double blind study is to prove that irradiation of a small area of the human body with visible and infrared polarized (VIP) light (400-3400 nm, 95% polarization, 40 mW cm(2), 12 J cm(2)) leads to an increase of the growth promoting (GP) activity of the entire circulating blood for primary cultures of human keratinocytes (KCs). Thirty minutes after the VIP-irradiation of a sacral area of volunteers, the GP activity of circulating blood was seen to increase through the elevation of the number of KCs cultured with the isolated plasma by 20 +/- 3%, p < 0.001. A similar increase in GP activity was seen in plasma derived from the in vitro irradiated blood of each volunteer, and from the mixture of irradiated and non-irradiated autologous blood 1:10. Enhanced GP activity was also recorded at 24 h after the 1st and 4-9th daily phototherapeutic sessions. Hence, exposure of volunteers to VIP light leads to a fast increase in the GP activity of the entire circulating blood for human KCs in vitro, which is a consequence of the transcutaneous photomodification of blood and its effect on the rest of the circulating blood volume.
低强度激光促进伤口愈合的全身机制在很大程度上仍未得到研究。本随机、安慰剂对照、双盲研究的目的是证明，用可见和红外偏振（VIP）光（400 - 3400 nm，95%偏振，40 mW/cm²，12 J/cm²）照射人体小面积区域会导致人角质形成细胞（KC）原代培养中全循环血液的生长促进（GP）活性增加。在志愿者骶部区域接受VIP照射30分钟后，通过分离血浆培养的KC数量增加20±3%，观察到循环血液的GP活性增加，p < 0.001。在每位志愿者体外照射血液的血浆以及照射与未照射自体血液1:10的混合物中也观察到类似的GP活性增加。在第1次和第4 - 9次每日光疗疗程后24小时也记录到GP活性增强。因此，志愿者暴露于VIP光会导致体外人KC全循环血液的GP活性快速增加，这是血液经皮光调制及其对其余循环血量影响的结果。
