通过图像分析观察大量单个细菌的生长和分裂。
Observing growth and division of large numbers of individual bacteria by image analysis.
作者信息
Elfwing A, LeMarc Y, Baranyi J, Ballagi A
机构信息
Center for Surface Biotechnology, University of Uppsala, S-751 23 Uppsala, Sweden.
出版信息
Appl Environ Microbiol. 2004 Feb;70(2):675-8. doi: 10.1128/AEM.70.2.675-678.2004.
Abstract
We describe a method that enabled us to observe large numbers of individual bacterial cells during a long period of cell growth and proliferation. We designed a flow chamber in which the cells attached to a transparent solid surface. The flow chamber was mounted on a microscope equipped with a digital camera. The shear force of the flow removed the daughter cells, making it possible to monitor the consecutive divisions of a single cell. In this way, kinetic parameters and their distributions, as well as some physiological characteristics of the bacteria, could be analyzed based on more than 1,000 single-cell observations. The method which we developed enabled us to study the history effect on the distribution of the lag times of single cells.
摘要
我们描述了一种方法，该方法使我们能够在细胞长时间生长和增殖过程中观察大量单个细菌细胞。我们设计了一个流动腔室，细胞附着在该腔室的透明固体表面上。流动腔室安装在配备有数码相机的显微镜上。流动产生的剪切力会移除子细胞，从而能够监测单个细胞的连续分裂。通过这种方式，可以基于1000多个单细胞观察结果来分析动力学参数及其分布，以及细菌的一些生理特征。我们开发的这种方法使我们能够研究历史效应对单个细胞延迟期分布产生的影响。
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