Ultee A, Souvatzi N, Maniadi K, König H
Institute of Microbiology and Wine Research, Mainz, Germany.
J Appl Microbiol. 2004;96(3):560-8. doi: 10.1111/j.1365-2672.2004.02174.x.
The identification of the culturable and nonculturable bacterial population in ground water of a municipal water supply in Mainz (Germany) during the year 2002.
Total counts varied between 3.5 x 103 and 2.2 x 104 cells ml-1, viable counts were approximately between 8.1 x 102 and 3.3 x 103 cells ml-1. After cultivation on different nutrient media (R2A, DEV, PCA, Endo, Standard) <1% appeared to be culturable on the media used. After denaturating gradient gel electrophoreses, up to 24 different bacterial species were detected in the ground water. With the aid of 16S rDNA isolation, amplification and sequencing, the isolated organisms and clones could be identified.
The isolated and cultured organisms mainly belonged to the Proteobacteria (alpha, beta and gamma), Flavobacteria or Actinobacteria. However, most of the noncultured micro-organisms were beta-Proteobacteria.
This is the first study in which the identification of all culturable and nonculturable bacteria in a ground water has been attempted.
鉴定2002年德国美因茨市公共供水地下水中可培养和不可培养的细菌菌群。
总菌数在3.5×10³至2.2×10⁴个细胞/毫升之间变化，活菌数约在8.1×10²至3.3×10³个细胞/毫升之间。在不同营养培养基（R2A、DEV、PCA、Endo、标准培养基）上培养后，在所使用的培养基上<1%的细菌似乎是可培养的。经过变性梯度凝胶电泳后，在地下水中检测到多达24种不同的细菌种类。借助16S rDNA的分离、扩增和测序，可以鉴定出分离出的生物体和克隆。
分离和培养出的生物体主要属于变形菌门（α、β和γ）、黄杆菌属或放线菌门。然而，大多数未培养的微生物是β-变形菌。
这是首次尝试鉴定地下水中所有可培养和不可培养细菌的研究。
