Suontaka A M, Bremme K, Akerblom O, Blombäck M
Department of Immunology, Karolinska Hospital, Stockholm, Sweden.
Infusionsther Transfusionsmed. 1992 Jun;19(3):110-4. doi: 10.1159/000222598.
To maintain a closed system during the preparation of blood components, including the removal of buffy coat, many centers use a quadruple blood bag additive solution system which in this study has been reduced to a cheaper triple bag system. The buffy coat and plasma were after centrifugation transferred to the first satellite bag and, after a second spin, the plasma separated from the buffy coat was transferred to the second satellite bag and stored for a fortnight at 4 degrees C. This resulted in a statistically significant increase in platelet factor 4 and elastase activity levels. No significant changes were found in the levels of C1-esterase inhibitor and kallikrein inhibiting activity, thrombin-antithrombin complexes, soluble fibrin, fibrinopeptide A and spontaneous proteolytic activity. The changes observed must be regarded as clinically insignificant. The platelet count is low enough to meet the requirements for platelet poor plasma. Using this blood component separation technique, one can reduce the CPD/additive solution 4-pack blood bag system to a less expensive 3-pack blood bag system.
为在制备血液成分（包括去除白膜层）过程中维持封闭系统，许多中心使用四联血袋添加剂溶液系统，而在本研究中已简化为成本更低的三联袋系统。离心后，白膜层和血浆被转移至第一个卫星袋，再次离心后，从白膜层分离出的血浆被转移至第二个卫星袋，并在4℃下储存两周。这导致血小板第4因子和弹性蛋白酶活性水平出现统计学上的显著升高。在C1酯酶抑制剂水平、激肽释放酶抑制活性、凝血酶 - 抗凝血酶复合物、可溶性纤维蛋白、纤维蛋白肽A和自发蛋白水解活性方面未发现显著变化。所观察到的这些变化在临床上可视为无意义。血小板计数低至足以满足少血小板血浆的要求。使用这种血液成分分离技术，可将CPD/添加剂溶液四袋血袋系统简化为成本较低的三袋血袋系统。
