Hanasono Matthew M, Lum Joanne, Carroll Lisa A, Mikulec Anthony A, Koch R James
Wound Healing and Tissue Engineering Laboratory, Division of Otolaryngology/Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, USA.
Arch Facial Plast Surg. 2004 Mar-Apr;6(2):88-93. doi: 10.1001/archfaci.6.2.88.
Topical silicone gel has shown promise in the treatment of hypertrophic and keloid scars. However, its mechanism of action remains undetermined.
To investigate whether the presence of silicone alters the secretion of basic fibroblast growth factor (bFGF), a key cytokine involved in the scar formation process.
Serum-free fibroblast cell cultures were established from normal, keloid, and fetal skin, which heals without scarring, and exposed to silicone gel. Serial cell counts were performed, and supernatants were collected for bFGF quantification by enzyme-linked immunosorbent assay at 4, 24, 72, and 120 hours.
Growth curves were similar and no statistically significant differences in population doubling times were observed between treated and untreated specimens. Statistically significant differences in bFGF levels between treated and untreated normal fibroblasts were observed at 24, 72, and 120 hours after cell culture initiation. Differences in bFGF levels between treated and untreated fetal fibroblasts that approached statistical significance were observed at 72 and 120 hours.
These results suggest that silicone gel is responsible for increased bFGF levels in normal and fetal dermal fibroblasts. We postulate that silicone gel treats and prevents hypertrophic scar tissue, which contains histologically normal fibroblasts, by modulating expression of growth factors such as bFGF. Our data support the hypothesis that substances that favorably influence wound healing do so by correcting a deficiency or overabundance of the growth factors that orchestrate the tissue repair process.
外用硅胶凝胶在治疗增生性瘢痕和瘢痕疙瘩方面已显示出前景。然而，其作用机制仍未明确。
研究硅胶的存在是否会改变碱性成纤维细胞生长因子（bFGF）的分泌，bFGF是瘢痕形成过程中一种关键的细胞因子。
从正常皮肤、瘢痕疙瘩皮肤和无瘢痕愈合的胎儿皮肤中建立无血清成纤维细胞培养物，并使其接触硅胶凝胶。进行连续细胞计数，并在4、24、72和120小时收集上清液，通过酶联免疫吸附测定法对bFGF进行定量。
生长曲线相似，处理组和未处理组标本之间在群体倍增时间上未观察到统计学上的显著差异。在细胞培养开始后的24、72和120小时，观察到处理组和未处理组正常成纤维细胞之间bFGF水平存在统计学上的显著差异。在72和120小时，观察到处理组和未处理组胎儿成纤维细胞之间bFGF水平的差异接近统计学显著性。
这些结果表明硅胶凝胶可导致正常和胎儿真皮成纤维细胞中bFGF水平升高。我们推测硅胶凝胶通过调节bFGF等生长因子的表达来治疗和预防增生性瘢痕组织，该组织含有组织学上正常的成纤维细胞。我们的数据支持这样的假设，即对伤口愈合有有利影响的物质是通过纠正协调组织修复过程的生长因子的缺乏或过量来实现的。
