Wang L, Reipa V, Blasic J
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899-8312, USA.
Bioconjug Chem. 2004 Mar-Apr;15(2):409-12. doi: 10.1021/bc030047k.
We successfully conjugated 1-2 nm diameter silicon nanoparticles to a 5'-amino-modified oligonucleotide (60mer) that contains a C6 linker between amide and phosphate groups. The conjugation was implemented via two photoinduced reactions followed by a DNA labeling step through formation of a carboxamide bond. Photoluminescence of the conjugates is dominated by two blue bands (400 and 450 nm maximal) under 340 nm excitation. The quantum yield of oligonucleotide-conjugated nanoparticles was determined to be 0.08 as measured against quinine sulfate in 0.1 M HClO(4) as a reference standard. We report a conjugation process that allows labeling of Si nanoparticles to an oligonucleotide in aqueous solutions. Ways to further optimize the procedure in order to achieve narrower and brighter photoluminescence are discussed.
我们成功地将直径为1-2纳米的硅纳米颗粒与一种5'-氨基修饰的寡核苷酸（60聚体）偶联，该寡核苷酸在酰胺基和磷酸基团之间含有一个C6连接子。偶联是通过两个光诱导反应实现的，随后通过形成羧酰胺键进行DNA标记步骤。在340纳米激发下，偶联物的光致发光由两个蓝色带（最大波长分别为400和450纳米）主导。以0.1 M高氯酸中的硫酸奎宁作为参考标准，测定寡核苷酸偶联纳米颗粒的量子产率为0.08。我们报道了一种在水溶液中将硅纳米颗粒标记到寡核苷酸上的偶联过程。还讨论了进一步优化该过程以实现更窄且更亮的光致发光的方法。
