Department of Chemistry and Biochemistry, Materials Science and Engineering Program, University of California, San Diego, 9500 Gilman, La Jolla, CA 92093, USA.
Small. 2010 Nov 22;6(22):2546-52. doi: 10.1002/smll.201000841.
Magnetic manipulation, fluorescent tracking, and localized delivery of a drug payload to cancer cells in vitro is demonstrated, using nanostructured porous silicon microparticles as a carrier. The multifunctional microparticles are prepared by electrochemical porosification of a silicon wafer in a hydrofluoric acid-containing electrolyte, followed by removal and fracture of the porous layer into particles using ultrasound. The intrinsically luminescent particles are loaded with superparamagnetic iron oxide nanoparticles and the anti-cancer drug doxorubicin. The drug-containing particles are delivered to human cervical cancer (HeLa) cells in vitro, under the guidance of a magnetic field. The high concentration of particles in the proximity of the magnetic field results in a high concentration of drug being released in that region of the Petri dish, and localized cell death is confirmed by cellular viability assay (Calcein AM).
采用纳米结构多孔硅微球作为载体，实现了对体外癌细胞的磁性操纵、荧光跟踪和药物有效负载的定位输送。多功能微球是通过在含有氢氟酸的电解液中电化学渗透硅片制备的，然后通过超声将多孔层去除并断裂成颗粒。本征发光颗粒负载超顺磁性氧化铁纳米颗粒和抗癌药物阿霉素。在磁场的引导下，将含药物的颗粒递送到体外人宫颈癌（HeLa）细胞中。在磁场附近的高浓度颗粒导致药物在培养皿的该区域的高浓度释放，并通过细胞活力测定（Calcein AM）证实局部细胞死亡。
