Wang S T, Demshar H P
Chemistry Section, Ontario Ministry of Health, Etobicoke, Canada.
Analyst. 1992 Jun;117(6):959-61. doi: 10.1039/an9921700959.
A simple procedure for the determination of blood-lead levels in dried blood-spot filter-paper specimens is described. A 3/16 inch dried blood spot was analysed by a method involving extraction of the lead into 1.25% (NH4)2HPO4-0.5% Triton X-100 solution. A matrix-based calibration was used for analysis, with use of a Zeeman-effect background corrected atomic absorption spectrometer. The within-run precision (% relative standard deviation) of the method at the low end of the analytical range was 19% at 0.36 mumol dm-3 and 14% at 0.60 mumol dm-3. The accuracy of the method was verified by recovery tests and by comparison with a routine whole-blood method. The mean blood lead level of 425 Toronto newborns was 0.19 mumol dm-3 (range 0-0.75 mumol dm-3).
本文描述了一种用于测定干血斑滤纸样本中血铅水平的简单方法。采用一种将铅提取到1.25%（NH4）2HPO4 - 0.5% Triton X - 100溶液中的方法，对直径为3/16英寸的干血斑进行分析。分析采用基于基质的校准，使用塞曼效应背景校正原子吸收光谱仪。该方法在分析范围低端的批内精密度（相对标准偏差百分比）在0.36 μmol dm-3时为19%，在0.60 μmol dm-3时为14%。通过回收率测试和与常规全血方法比较验证了该方法的准确性。425名多伦多新生儿的平均血铅水平为0.19 μmol dm-3（范围为0 - 0.75 μmol dm-3）。
