Hammer M, Nagel E, Schweitzer D, Richter S, Schweitzer F, Königsdörffer E, Strobel J
Augenklinik, Friedrich-Schiller-Universität, Jena.
Ophthalmologe. 2004 Dec;101(12):1189-93. doi: 10.1007/s00347-004-1019-z.
Alterations in the intrinsic ocular fundus fluorescence are under discussion particularly in connection with age-related macular degeneration. However, further knowledge of the chemical substrate of fluorescence is necessary.
Ocular fundus autofluorescence was observed using a fundus camera. The fluorescence emission was recorded using a 3-chip CCD camera. For comparison, the fluorescence spectra of lipofuscin, A2-E, FAD, NADH(2) and AGE's were recorded by the Jena ophthalmo-spectrometer.
In contrast to the homogeneous intrinsic fluorescence of a normal fundus, the fluorescence images of patients suffering from AMD showed remarkable local differences. The detected fluorescence spectra showed remarkable overlaps.
We introduced a new technique enabling the detection of spectral differences in images of ocular fundus autofluorescence. Besides the fluorescence of lipofuscin, we found a green one in patients suffering from nonexudative AMD.
眼内固有荧光的改变，尤其是与年龄相关性黄斑变性相关的改变，正受到讨论。然而，有必要进一步了解荧光的化学底物。
使用眼底照相机观察眼底自发荧光。使用三芯片电荷耦合器件（CCD）相机记录荧光发射。为作比较，用耶拿眼科光谱仪记录脂褐素、A2-E、黄素腺嘌呤二核苷酸（FAD）、还原型烟酰胺腺嘌呤二核苷酸（NADH(2)）和晚期糖基化终末产物（AGE's）的荧光光谱。
与正常眼底均匀的固有荧光不同，年龄相关性黄斑变性患者的荧光图像显示出明显的局部差异。检测到的荧光光谱显示出明显的重叠。
我们引入了一种新技术，能够检测眼底自发荧光图像中的光谱差异。除了脂褐素的荧光外，我们在非渗出性年龄相关性黄斑变性患者中发现了一种绿色荧光。
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