Beasley David W C, Holbrook Michael R, Travassos Da Rosa Amelia P A, Coffey Lark, Carrara Anne-Sophie, Phillippi-Falkenstein Kathrine, Bohm Rudolf P, Ratterree Marion S, Lillibridge Kristy M, Ludwig George V, Estrada-Franco Jose, Weaver Scott C, Tesh Robert B, Shope Robert E, Barrett Alan D T
Department of Pathology, UTMB, 301 University Blvd., Galveston, TX 77555-0609, USA.
J Clin Microbiol. 2004 Jun;42(6):2759-65. doi: 10.1128/JCM.42.6.2759-2765.2004.
Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.
西尼罗河病毒(WNV)感染的血清学诊断因与其他密切相关的黄病毒(如圣路易斯脑炎病毒)存在广泛的抗原交叉反应而变得复杂。在此,我们描述了一种重组的、细菌表达的抗原,其等同于WNV包膜蛋白的结构域III,在使用标准化对照抗血清和现场采集样本的间接酶联免疫吸附试验中,该抗原能够清晰地区分针对WNV的抗体反应与针对其他相关黄病毒的抗体反应。