Tailor Dharmesh R, Baumgardner James E, Regatte Ravinder R, Leigh John S, Reddy Ravinder
University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
Neuroimage. 2004 Jun;22(2):611-8. doi: 10.1016/j.neuroimage.2004.02.024.
In vivo detection of H(2)(17)O produced via metabolic reduction of inhaled (17)O-enriched gas is demonstrated using proton magnetic resonance imaging (MRI). Specifically, (1)H T(1rho)-weighted MRI, which may be readily implemented on any MRI scanner, is applied as an indirect (17)O imaging method to quantitatively monitor the distribution of metabolically produced (17)O water (mpH(2)(17)O) in the rat brain. The delivery of (17)O(2) to rats is conducted via a specially designed closed respiration circuit that conserves the expensive gas. Quantitative mapping of H(2)(17)O performed via (1)H T(1rho)-weighted MRI is validated by direct (17)O-magnetic resonance spectroscopy. The MRI data show that a steady-state H(2)(17)O concentration of 25.7 +/- 1.66 mM (n = 4) is achieved in the rat brain within approximately 30 min under the (17)O inhalation paradigm used. From the first minute of the mpH(2)(17)O time courses, cerebral metabolic rate of oxygen (CMRO(2)) is estimated to be 2.10 +/- 0.44 micromol g(-1) min(-1) (n = 4), a value that is consistent with the literature.
利用质子磁共振成像（MRI）在体内检测通过吸入富含(^{17}O)的气体代谢还原产生的(H_2^{17}O)。具体而言，可在任何MRI扫描仪上轻松实现的(^1H T_1\rho)加权MRI被用作间接(^{17}O)成像方法，以定量监测大鼠脑中代谢产生的(^{17}O)水（(mpH_2^{17}O)）的分布。通过专门设计的封闭呼吸回路将(^{17}O_2)输送给大鼠，该回路可保存昂贵的气体。通过(^1H T_1\rho)加权MRI对(H_2^{17}O)进行的定量映射通过直接(^{17}O)磁共振波谱法得到验证。MRI数据表明，在所用的(^{17}O)吸入模式下，大鼠脑内约30分钟内达到的稳态(H_2^{17}O)浓度为(25.7 \pm 1.66 mM)（(n = 4)）。从(mpH_2^{17}O)时间进程的第一分钟起，估计脑氧代谢率（(CMRO_2)）为(2.10 \pm 0.44)微摩尔(g^{-1})分钟(^{-1})（(n = 4)），该值与文献一致。
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