基于共振光散射增强法用三唑醇测定脱氧核糖核酸

The determination of deoxyribonucleic acids with triadimenol based on the enhancement of resonance light scattering.

作者信息

Jie Nianqin, Hou Shicong, Du Fengpei, Zhang Chunrong, Qin Guoxin

机构信息

College of Applied Chemistry, China Agricultural University, Beijing, PR China.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2004;23(4):725-34. doi: 10.1081/NCN-120037750.

DOI:10.1081/NCN-120037750

PMID:15200034

Abstract

For the first time, triadimenol was used to determine nucleic acid (DNA) using the resonance light scattering (RLS) technique. The RLS of triadimenol was greatly enhanced by DNA in the range of pH 1.6 to approximately 1.9. A resonance light-scattering peak at 310 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0 to approximately 9 microg/ml with the detection limit of 24 ng ml(-1). The mechanism studies of the system indicated that the enhanced RLS is due to the aggregation of triadimenol on DNA. The nucleic acids in synthetic samples and in rice seedling extraction were analyzed with satisfactory results. Compared with other methods, this method is convenient, rapid, inexpensive and simple.

摘要

首次使用三唑醇通过共振光散射（RLS）技术测定核酸（DNA）。在pH 1.6至约1.9范围内，DNA极大地增强了三唑醇的共振光散射。发现了一个在310 nm处的共振光散射峰，该波长下共振光散射增强的强度与DNA浓度成正比。校准曲线的线性范围为0至约9 μg/ml，检测限为24 ng/ml。该体系的机理研究表明，共振光散射增强是由于三唑醇在DNA上的聚集。对合成样品和水稻幼苗提取物中的核酸进行了分析，结果令人满意。与其他方法相比，该方法方便、快速、廉价且简单。