[用玫瑰苯胺通过共振光散射法测定脱氧核糖核酸]
[Determination of deoxyribonucleic acid with rosaniline by a resonance light scattering method].
作者信息
Liu C, Chen X M, Xiang H Y, Li S, Xia S, Liu A X
机构信息
Institute of Chemistry and Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
出版信息
Guang Pu Xue Yu Guang Pu Fen Xi. 2001 Oct;21(5):697-700.
The resonance light scattering (RLS) spectra of Rosaniline with DNA have been studied. The RLS of Rosaniline is greatly enhanced by DNA in pH range of 10.5-10.8. There is a resonance light scattering peak ofat 485 nm, and the enhanced intensity of RLS at this wavelength is proportional to the concentration of DNA. The linear range of the calibration graph is 0-1.00 mg.L-1 with the correlation coefficient of 0.9980. The detection limit is 14.2 ng.mL-1. The relative standard deviation(n = 10) was within 2.1%. This method is simple, rapid and has been applied to the determination of DNA in mixed samples with satisfactory results.
研究了玫瑰苯胺与DNA的共振光散射（RLS）光谱。在pH值为10.5 - 10.8的范围内，DNA能极大地增强玫瑰苯胺的RLS。在485nm处有一个共振光散射峰，该波长下RLS增强强度与DNA浓度成正比。校准曲线的线性范围为0 - 1.00mg·L⁻¹，相关系数为0.9980。检测限为14.2ng·mL⁻¹。相对标准偏差（n = 10）在2.1%以内。该方法简单、快速，已应用于混合样品中DNA的测定，结果令人满意。
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