Marsili I, Pizza M, Giovannoni F, Volpini G, Bartalini M, Olivieri R, Rappuoli R, Nencioni L
Sclavo Research Center, Siena, Italy.
Infect Immun. 1992 Mar;60(3):1150-5. doi: 10.1128/iai.60.3.1150-1155.1992.
Bordetella pertussis 165-9K/129G, which produces a nontoxic form of pertussis toxin (PT), was used to prepare a whole-cell diphtheria-tetanus-pertussis (DTP) vaccine. The in vivo potency and the serological response induced by this vaccine were comparable to those of the conventional DTP vaccine which contains active PT. The toxic activities induced by PT such as leukocytosis, histamine sensitivity, and potentiation of anaphylactic reactions, which are present in the conventional DTP vaccine, were absent in the new vaccine. These results suggest that the introduction of a whole-cell vaccine containing B. pertussis 165-9K/129G would induce the same immunity as the conventional vaccine and would avoid the administration of a harmful toxin to children.
百日咳博德特氏菌165 - 9K/129G可产生无毒形式的百日咳毒素（PT），用其制备了全细胞白喉-破伤风-百日咳（DTP）疫苗。该疫苗诱导的体内效力和血清学反应与含有活性PT的传统DTP疫苗相当。传统DTP疫苗中存在的由PT诱导的毒性活性，如白细胞增多、组胺敏感性和过敏反应增强，在新疫苗中不存在。这些结果表明，引入含有百日咳博德特氏菌165 - 9K/129G的全细胞疫苗将诱导与传统疫苗相同的免疫力，并避免给儿童施用有害毒素。
