Posada A B, Jonasson J, de Linares L, Bygdeman S
Department of Microbiology, Faculty of Medicine, University of El Salvador.
Int J STD AIDS. 1992 Jan-Feb;3(1):33-7. doi: 10.1177/095646249200300108.
One-hundred and twenty-nine pregnant women in labour (age range 15-46 years; median age 23) and 42 infants born to chlamydia-positive mothers (age range 5-15 days; median age 10) were investigated to estimate the prevalence and incidence, respectively, of Chlamydia trachomatis infection in San Salvador, El Salvador. Urethral and cervical samples were obtained from all women and conjunctival specimens were taken from both eyes of each child. The chlamydial antigen was detected with the commercial Pharmacia Chlamydia EIA kit. Direct immunofluorescence (DFA) (Syva MicroTrak) was used for confirmation. In the newborns both EIA and DFA tests on direct preparations from ocular smears were performed on all the samples. The prevalence of chlamydial infection in pregnant women was 44% (57/129). The incidence of chlamydial infection in neonates was 64% (27/42), and the majority of the infected children (56%) had conjunctivitis. Referring to individuals rather than specimens the sensitivity of EIA tests on conjunctival samples from the infants was low (37%) as compared with 91% on urethral and cervical specimens from the pregnant women.
对129名分娩期孕妇（年龄范围15 - 46岁；中位年龄23岁）和42名母亲衣原体检测呈阳性的婴儿（年龄范围5 - 15天；中位年龄10天）进行了调查，以分别估算萨尔瓦多圣萨尔瓦多沙眼衣原体感染的患病率和发病率。从所有女性身上采集尿道和宫颈样本，并从每个孩子的双眼采集结膜标本。使用商业化的法玛西亚衣原体酶免疫分析试剂盒检测衣原体抗原。采用直接免疫荧光法（DFA）（Syva MicroTrak）进行确认。对于新生儿，对所有样本的眼部分泌物直接涂片同时进行酶免疫分析和直接免疫荧光检测。孕妇中衣原体感染的患病率为44%（57/129）。新生儿衣原体感染的发病率为64%（27/42），且大多数受感染儿童（56%）患有结膜炎。以个体而非标本为参照，对婴儿结膜样本进行酶免疫分析检测的灵敏度较低（37%），而对孕妇尿道和宫颈标本进行酶免疫分析检测的灵敏度为91%。
