三裂叶野海棠中琼苷D的抗氧化活性

Antioxidant activity of jionoside D from Clerodendron trichotomum.

作者信息

Chae Sungwook, Kim Ju Sun, Kang Kyoung Ah, Bu Hyung Deuk, Lee Youngki, Hyun Jin Won, Kang Sam Sik

机构信息

Natural Products Research Institute, Seoul National University, Yeunkun dong, Chongno ku, Korea.

出版信息

Biol Pharm Bull. 2004 Oct;27(10):1504-8. doi: 10.1248/bpb.27.1504.

DOI:10.1248/bpb.27.1504

PMID:15467185

Abstract

The antioxidant property of jionoside D, isolated from Clerodendron trichotomum (Verbenaceae), was investigated. This compound showed scavenging activity of intracellular reactive oxygen species and of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, as well as lipid peroxidation inhibitory activity. This radical scavenging activity of jionoside D protected the cell viability of Chinese hamster lung fibroblast (V79-4) cells exposed H2O2. Furthermore, jionoside D reduced the apoptotic cells induced by H2O2, as demonstrated by the decreased number of sub G1 hypo-diploid cells and apoptotic body formation. However, it increased the activities of cellular antioxidant enzymes, superoxide dismutase and catalase. Taken together, these findings suggest that jionoside D, isolated from C. trichotomum, exhibits antioxidant properties.

摘要

研究了从海州常山（马鞭草科）中分离得到的海州常山苷D的抗氧化特性。该化合物表现出对细胞内活性氧和1,1-二苯基-2-苦基肼（DPPH）自由基的清除活性，以及脂质过氧化抑制活性。海州常山苷D的这种自由基清除活性保护了暴露于H2O2的中国仓鼠肺成纤维细胞（V79-4）的细胞活力。此外，海州常山苷D减少了由H2O2诱导的凋亡细胞，这通过亚G1期亚二倍体细胞数量的减少和凋亡小体的形成得以证明。然而，它增加了细胞抗氧化酶超氧化物歧化酶和过氧化氢酶的活性。综上所述，这些发现表明从海州常山分离得到的海州常山苷D具有抗氧化特性。