OXA-60，一种来自皮氏罗尔斯通氏菌的染色体介导的、可诱导的、能水解亚胺培南的D类β-内酰胺酶。
OXA-60, a chromosomal, inducible, and imipenem-hydrolyzing class D beta-lactamase from Ralstonia pickettii.
作者信息
Girlich Delphine, Naas Thierry, Nordmann Patrice
机构信息
Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cédex, France.
出版信息
Antimicrob Agents Chemother. 2004 Nov;48(11):4217-25. doi: 10.1128/AAC.48.11.4217-4225.2004.
Abstract
A chromosomally encoded oxacillinase, OXA-22, had been characterized from Ralstonia pickettii PIC-1 that did not explain by itself the resistance profile of this strain to beta-lactams. Thus, further analysis of the genetic background of this species led to the identification of another oxacillinase, OXA-60, that was expressed only after beta-lactam induction. This chromosomally encoded oxacillinase shared 19% amino acid identity with OXA-22. It has a narrow-spectrum hydrolysis profile that includes imipenem. OXA-60-like enzymes were identified in several R. pickettii strains. Gene inactivation and induction studies of the bla(OXA-60) and bla(OXA-22) genes in R. pickettii identified the relative contribution of each oxacillinase to the resistance profile of R. pickettii to beta-lactams.
摘要
一种染色体编码的苯唑西林酶OXA - 22已从皮氏罗尔斯顿菌PIC - 1中得到鉴定，但其本身并不能解释该菌株对β-内酰胺类抗生素的耐药谱。因此，对该菌种遗传背景的进一步分析导致了另一种苯唑西林酶OXA - 60的鉴定，该酶仅在β-内酰胺诱导后表达。这种染色体编码的苯唑西林酶与OXA - 22具有19%的氨基酸同一性。它具有窄谱水解谱，包括亚胺培南。在几株皮氏罗尔斯顿菌中鉴定出了OXA - 60样酶。对皮氏罗尔斯顿菌中bla(OXA - 60)和bla(OXA - 22)基因的基因失活和诱导研究确定了每种苯唑西林酶对皮氏罗尔斯顿菌对β-内酰胺类抗生素耐药谱的相对贡献。
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