Kadosh David, Johnson Alexander D
Department of Microbiology and Immunology, University of California-San Francisco, San Francisco, CA 94143-2200, USA.
Mol Biol Cell. 2005 Jun;16(6):2903-12. doi: 10.1091/mbc.e05-01-0073. Epub 2005 Apr 6.
Candida albicans, the major human fungal pathogen, undergoes a reversible morphological transition from blastospores (round budding cells) to filaments (elongated cells attached end-to-end). This transition, which is induced upon exposure of C. albicans cells to a number of host conditions, including serum and body temperature (37 degrees C), is required for virulence. Using whole-genome DNA microarray analysis, we describe 61 genes that are significantly induced (> or =2-fold) during the blastospore to filament transition that takes place in response to exposure to serum and 37 degrees C. We next show that approximately half of these genes are transcriptionally repressed in the blastospore state by three transcriptional repressors, Rfg1, Nrg1, and Tup1. We conclude that the relief of this transcriptional repression plays a key role in bringing the C. albicans filamentous growth program into play, and we describe the framework of this transcriptional circuit.
白色念珠菌是主要的人类真菌病原体，它会经历从芽生孢子（圆形出芽细胞）到菌丝（首尾相连的细长细胞）的可逆形态转变。这种转变在白色念珠菌细胞暴露于多种宿主条件下时被诱导，包括血清和体温（37摄氏度），是其毒力所必需的。通过全基因组DNA微阵列分析，我们描述了61个在芽生孢子到菌丝转变过程中显著上调（≥2倍）的基因，这种转变是在暴露于血清和37摄氏度时发生的。接下来我们表明，这些基因中大约一半在芽生孢子状态下被三种转录抑制因子Rfg1、Nrg1和Tup1转录抑制。我们得出结论，这种转录抑制的解除在启动白色念珠菌丝状生长程序中起关键作用，并且我们描述了这个转录回路的框架。
