Willett R L, Baldwin K W, West K W, Pfeiffer L N
Bell Laboratories, Lucent Technologies, 600 Mountain Avenue, Murray Hill, NJ 07974, USA.
Proc Natl Acad Sci U S A. 2005 May 31;102(22):7817-22. doi: 10.1073/pnas.0408565102. Epub 2005 May 18.
A fundamental, yet underexplored, materials system is the interface between biological molecules and inorganic surfaces. In an elemental approach to this problem, we have systematically examined the adhesion of amino acids to a series of inorganic surfaces including metals, insulators, and semiconductors. Significant differential adhesion is observed over the full complement of amino acids, determined largely by amino acid side-chain charge. Extensive mapping of the amino acid adhesion versus materials in multiple solutions is presented, with preliminary mechanisms derived from concentration and pH dependence. These results provide an empirical basis for building peptide to inorganic surface structures, and, using this adhesion data, we design inorganic nanostructures that are shown to selectively bind to prescribed primary peptide sequences.
一个基本但尚未得到充分探索的材料体系是生物分子与无机表面之间的界面。在解决这个问题的基本方法中，我们系统地研究了氨基酸在包括金属、绝缘体和半导体在内的一系列无机表面上的附着力。在整个氨基酸范围内观察到显著的差异附着力，这在很大程度上由氨基酸侧链电荷决定。本文展示了在多种溶液中氨基酸附着力与材料之间的广泛映射，并从浓度和pH依赖性推导出初步机制。这些结果为构建肽与无机表面结构提供了经验基础，并且利用这些附着力数据，我们设计出了被证明能选择性结合特定一级肽序列的无机纳米结构。
