Poggio Claudio, Lombardini Marco, Colombo Marco, Dagna Alberto, Saino Enrica, Arciola Carla Renata, Visai Livia
Department of Endodontics, University of Pavia, Pavia, Italy.
Int J Artif Organs. 2011 Sep;34(9):908-13. doi: 10.5301/ijao.5000055.
The objective of this study was to perform an in vitro evaluation of the antibacterial properties of 6 endodontic sealers (Endomethasone C, Argoseal, Bioseal Normal, Acroseal, AH Plus, Sicura Seal).
The agar diffusion test (well and paper disc methods) with Enterococcus faecalis, Staphylococcus aureus and Streptococcus mutans was used. For the well method, Petri dishes were inoculated with bacterial suspensions. Each well was completely filled with freshly mixed endodontic sealer. For the paper disc method, sterile paper discs were immersed in freshly mixed sealers and put on agar plates. Diameters of halos formed around the sealers were measured after 24 h and 48 h.
The effects of well method and of paper disc method were analyzed by 1-way ANOVA.
Endomethasone C, Argoseal and Bioseal showed the largest inhibition halos for all the tested microorganisms, while Sicura Seal and AH Plus showed low antibacterial effects. Moreover, the comparison of well method and paper disc methods showed significant statistical differences (P<0,01) for all sealers and indicated a dose-dependent antimicrobial effect.
本研究的目的是对6种根管封闭剂（Endomethasone C、Argoseal、Bioseal Normal、Acroseal、AH Plus、Sicura Seal）的抗菌性能进行体外评估。
采用琼脂扩散试验（孔板法和纸片法）检测粪肠球菌、金黄色葡萄球菌和变形链球菌。对于孔板法，在培养皿中接种细菌悬液。每个孔完全填满新鲜混合的根管封闭剂。对于纸片法，将无菌纸片浸入新鲜混合的封闭剂中，然后放在琼脂平板上。24小时和48小时后测量封闭剂周围形成的抑菌圈直径。
采用单因素方差分析孔板法和纸片法的效果。
Endomethasone C、Argoseal和Bioseal对所有测试微生物显示出最大的抑菌圈，而Sicura Seal和AH Plus显示出较低的抗菌效果。此外，孔板法和纸片法的比较显示，所有封闭剂均有显著统计学差异（P<0.01），表明抗菌作用呈剂量依赖性。
