Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason D Y
LRF Immunodiagnostics Unit, John Radcliffe Hospital, Headington, Oxford, U.K.
Immunology. 1992 Apr;75(4):588-95.
A new monoclonal antibody, GHI/61, which labels the majority of monocytes and tissue macrophages is described. This antibody recognizes an intracellular antigen of 130,000 MW (reduced) and 110,000 MW (unreduced). Using biochemical, flow cytometry and immunocytochemical studies antibody GHI/61 was shown to recognize the same antigen as the previously described antibodies Ber-MAC3, Ki-M8 and SM4. On the basis of the results obtained, antibodies GHI/61, SM4, Ber-MAC3 and Ki-M8 should form a new CD group at the next Leucocyte Typing Workshop. Since the antigen recognized by the antibody GHI/61 is relatively easy to purify, the sequencing and the isolation of the gene encoding this protein should be possible.
本文描述了一种新的单克隆抗体GHI/61，它可标记大多数单核细胞和组织巨噬细胞。该抗体识别一种分子量为130,000（还原态）和110,000（非还原态）的细胞内抗原。通过生化、流式细胞术和免疫细胞化学研究表明，抗体GHI/61与先前描述的抗体Ber-MAC3、Ki-M8和SM4识别相同的抗原。根据所得结果，抗体GHI/61、SM4、Ber-MAC3和Ki-M8应在下一届白细胞分型研讨会上形成一个新的CD组。由于抗体GHI/61识别的抗原相对易于纯化，因此对编码该蛋白的基因进行测序和分离应该是可行的。
