Wang Cong, Kasuga Toshihiro, Nogami Masayuki
Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-Cho, Showa-Ku, Nagoya, 466-8555, Japan.
J Mater Sci Mater Med. 2005 Aug;16(8):739-44. doi: 10.1007/s10856-005-2611-8.
Macroporous calcium phosphate glass-ceramic with an initial glass composition of 60CaO.30P2O5.3TiO2.7Na2O in mol% was successfully prepared by sintering the mixture compact consisting of calcium phosphate glass and sucrose powders, which was formed using a two-step pressing technique. After burning off the sucrose phase, a 3D interconnected macroporous structure was formed in the sintered body, in which the skeleton consisting of the calcium phosphate glass-ceramic (including beta-calcium pyrophosphate and beta-tricalcium phosphate as the crystalline phases) was transformed from the initial glass during the sintering. The macropores with several hundred microns in diameter and the large interconnection size (approximately 100 microm), which result from the controllably large-sized sucrose particles and the hot-pressing at a little higher temperature than the sucrose's melting point, are believed to meet the requirements for cell adhesion and bone tissue regeneration well. Moreover, in vitro dissolution behavior study indicates that the calcium phosphate glass-ceramic is soluble to an acetic acid solution of pH 5-7. These, together with the simplicity and feasibility of the innovative fabrication method itself, show that the formed porous glass-ceramic has a promising potential for application to a scaffold for bone tissue engineering.
通过烧结由磷酸钙玻璃和蔗糖粉末组成的混合坯块成功制备了初始玻璃组成（摩尔百分比）为60CaO.30P₂O₅.3TiO₂.7Na₂O的大孔磷酸钙玻璃陶瓷，该混合坯块采用两步压制技术成型。烧掉蔗糖相后，在烧结体中形成了三维相互连通的大孔结构，其中由磷酸钙玻璃陶瓷（包括β - 焦磷酸钙和β - 磷酸三钙作为晶相）组成的骨架在烧结过程中由初始玻璃转变而来。直径数百微米且互连尺寸较大（约100微米）的大孔，是由可控的大尺寸蔗糖颗粒以及在略高于蔗糖熔点的温度下进行热压形成的，据信能很好地满足细胞黏附和骨组织再生的要求。此外，体外溶解行为研究表明，该磷酸钙玻璃陶瓷可溶解于pH值为5 - 7的醋酸溶液中。这些特性，连同创新制造方法本身的简单性和可行性，表明所形成的多孔玻璃陶瓷在骨组织工程支架应用方面具有广阔的潜在应用前景。
