Kakumu S, Fukatsu A, Shinagawa T, Kurokawa S, Kusakabe A
Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.
J Clin Pathol. 1992 May;45(5):408-11. doi: 10.1136/jcp.45.5.408.
To evaluate the role of local interleukin 6 (IL-6) in the pathogenesis of acute and chronic liver disease.
The cellular site of IL-6 in cryostat sections of liver from 31 patients with liver disease was examined using indirect immunofluorescence with a monoclonal antibody.
IL-6 staining in sinusoidal endothelial cells was very noticeable and diffusely distributed in the lobules of specimens of acute viral hepatitis. IL-6 expression in endothelial cells, particularly in necrotic areas of hepatocytes, was increased and was accompanied by enhanced expression in Kupffer cells. In contrast, IL-6 staining in infiltrating mononuclear cells was prominent in portal tracts, and the numbers of cytokine positive cells were greater in specimens of chronic active hepatitis compared with chronic persistent hepatitis. In non-specific reactive hepatitis intrahepatic expression of IL-6 was minimal, while in alcoholic liver fibrosis the cytokine distribution in the lobules was similar to that of acute viral hepatitis.
These results indicate that locally produced IL-6 contributes to the inflammatory process and immunological response in acute and chronic liver disease.
评估局部白细胞介素6（IL-6）在急慢性肝病发病机制中的作用。
采用单克隆抗体间接免疫荧光法检测31例肝病患者肝脏冰冻切片中IL-6的细胞定位。
在急性病毒性肝炎标本的小叶中，窦状内皮细胞中的IL-6染色非常明显且呈弥漫性分布。内皮细胞中IL-6的表达增加，尤其是在肝细胞坏死区域，同时库普弗细胞中的表达也增强。相比之下，浸润的单核细胞中的IL-6染色在门管区很突出，与慢性持续性肝炎相比，慢性活动性肝炎标本中细胞因子阳性细胞的数量更多。在非特异性反应性肝炎中，肝内IL-6的表达极少，而在酒精性肝纤维化中，小叶内细胞因子的分布与急性病毒性肝炎相似。
这些结果表明，局部产生的IL-6在急慢性肝病的炎症过程和免疫反应中起作用。
